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The most enduring and romantic legend of the Russian Revolution — that two children of Czar Nicholas II and his wife, Alexandra, survived the slaughter that killed the rest of their family — may finally be put to rest with the positive identification of bone fragments from a lonely Russian grave.

Newly analyzed DNA evidence from a grave discovered in 2007 near that of the czar proves that the bones are from Anastasia and her brother Alexi. A report on the analysis was published online Tuesday in the journal PLoS One.

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